Cast (in credits order)
Dakota Fanning .... Fern
Julia Roberts .... Charlotte A. Cavatica (voice)
Oprah Winfrey .... Gussy (voice)
Steve Buscemi .... Templeton (voice)
Kathy Bates .... Bitsy (voice)
John Cleese .... Samuel (voice)
Thomas Haden Church .... Brooks (voice)
Robert Redford .... Ike (voice)
Cedric the Entertainer .... Golly (voice)
Jane Sibbett .... Joy (voice)
Jennifer Garner .... Susy (voice)
Reba McEntire .... Betsy (voice)
André Benjamin .... Elwyn (voice)
Gary Basaraba .... Homer Zuckerman
Siobhan Fallon .... Mrs. Zuckerman (as Siobhan Fallon Hogan)
Kevin Anderson .... Mr. Arable
Essie Davis .... Mrs. Arable
Beau Bridges .... Dr. Dorian
Noel Ballantine .... Townsperson
Russell Bass .... Marching Band Member
Abraham Benrubi
Don Bridges .... Fair Announcer
Courtney Taylor Burness .... Additional (voice)
Louis Corbett .... Avery
John Cullum
Elle Fanning .... Fern's Granddaughter
Erica Jones .... Baby Spider #1 (voice)
Dominic Scott Kay .... Wilbur (voice)
Ella Scott Lynch .... Pretty Woman
Leslie Mann
Nate Mooney
Zoë Raye .... Baby spider, girl (voice)
Tequan Richmond .... Uncle
Jennessa Rose .... Baby Spider #2 (voice)
Afton Smith
Cameron Pinches .... Photographer (uncredited)